NAT3 Alleles in Non-human Eukaryotes

*NAT3* allele (haplotype)^(a)	Nucleotide change(s)^(b)	Amino acid change(s)^(b)	Organism	Literature
(MOUSE)Nat31*	Reference	Reference	Mouse (Mus musculus)	2-5^(c)
(MUSMC)Nat32*	238G>A 607,608 TG>CA	A80T W203Q	Mouse (Mus musculus castaneus)	4^(c)
(MUSSP)Nat33*	147A>G 162C>T 238G>A 295T>C 413C>T 511T>C 607,608 TG>CA 638G>A 796G>A 857T>C	Synonymous Synonymous A80T C99R T138I S171P W203Q R213Q V266I V286A	Mouse (Mus spretus)	4^(c)
(RAT)Nat31*	Reference	Reference	Rat (Rattus norvegicus)	6,7
(RAT)Nat32*	619G>T	A207S	Rat (Rattus norvegicus)	7

Footnotes

(a) The gene symbols are assigned according to the current guidelines of the NAT Gene Nomenclature Committee [1]. The alleles of rodents are written as Nat (first letter uppercase and second and third letter lowercase). The alleles of all other species are all uppercase (e.g. NAT).

(b) The position of SNPs in each ORF is determined relative to the A of the ATG translation initiation codon, which is always considered as number 1. The first methionine of each NAT protein is amino acid number 1, with polymorphic amino acid positions determined accordingly. The reference allele of each NAT3 gene (usually the wild type allele or the first allele identified in a specific organism) is assigned symbol NAT3*1.

(c) Studied western European house mouse (Mus musculus domesticus) laboratory strains: i) inbred C57Bl/6J [2-5], Balb/c [2], C3H/HeJ [3], 129/Ola [4], CBA [4], A/J [2-5] and A/HeJ [3], ii) outbred PO [4] and TO [4]. Also, the wild-derived inbred strains Mus musculus castaneus (southestern Asian house mouse) and Mus spretus (western wild mouse) [4].

For new submissions or enquiries, please contact Dr. Sotiria Boukouvala (sboukouv@mbg.duth.gr).

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Literature

[1] Hein, D.W.; Boukouvala, S.; Grant, D.M.; Minchin, R.F. and Sim, E. (2008) Changes in consensus arylamine N-acetyltransferase gene nomenclature. Pharmacogenet. Genomics 18(4), 367-368.

[2] Kelly, S.L. and Sim, E. (1994) Arylamine N-acetyltransferase in Balb/c mice: identification of a novel mouse isoenzyme by cloning and expression in vitro. Biochem. J. 302(2), 347-353.

[3] Fretland, A.J.; Doll, M.A.; Gray, K.; Feng, Y. and Hein, D.W. (1997) Cloning, expression and recombinant expression of NAT1, NAT2, and NAT3 derived from C3H/HeJ (rapid) and A/HeJ (slow) acetylator inbred mouse: Functional characterization of the activation and deactivation of aromatic amine carcinogens. Toxicol. Appl. Pharmacol. 142(2), 360-366.

[4] Boukouvala, S.; Price, N. and Sim, E. (2002) Identification and functional characterization of novel polymorphisms associated with the genes for arylamine N-acetyltransferases in mice. Pharmacogenetics 12(5), 385-394.

[5] Estrada-Rodgers, L.; Levy, G.N. and Weber, W.W. (1998) Substrate selectivity of mouse N-acetyltransferase 1, 2, and 3 expressed in COS-1 cells. Drug Metab. Dispos. 26(5), 502-505.

[6] Walraven, J.M.; Doll, M.A. and Hein, D.W. (2006) Identification and characterization of functional rat arylamine N-acetyltransferase 3: comparisons with rat arylamine N-acetyltransferases 1 and 2. J. Pharmacol. Exp. Ther. 319(1), 369-375.

[7] Walraven, J.M.; Barker, D.F.; Doll, M.A. and Hein, D.W. (2007) Tissue expression and genomic sequences of rat N-acetyltransferases rNat1, rNat2, rNat3, and Functional characterization of a novel rNat3*2 genetic variant. Toxicol. Sci. 99(2), 413-421.

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